Seven Sisters may refer to:
- The Pleiades, seven sisters who are companions of Artemis in Greek mythology
- The Pleiades, a star cluster named for the mythological characters
- Seven Sisters, a group of oil companies
- Seven Sisters schools, a group of American women's colleges
- Qi Qiao Jie, a Chinese holiday, sometimes called "Seventh Sister's Birthday"
- Seven Sisters, England (two places):
- Seven Sisters, Wales, a village in the south Wales
- The Seven Sisters, fictional characters from Dungeons & Dragons
- Seven Sisters, a mountain range in British Columbia, Canada